Sustainable Manufacturing and Foundry Practices
Sudip Chattopadhyay1
and Arnab Sarkar2
1Department of Biochemistry, All India Institute of Medical Sciences, Kalyani, West Bengal, India
2Department of Mechanical Engineering, Indian Institute of Technology (BHU) Varanasi, Uttar Pradesh, India
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Tea polyphenols are key bioactive constituents responsible for the health-promoting properties and quality attributes of tea. Rapid, reliable and cost-effective methods for their quantification are therefore essential for quality control and food analysis. In this work, a micro-fabricated disposable screen-printed electrochemical sensor was developed for the quantitative determination of tea polyphenols, using catechin as a representative standard compound. The electrochemical behaviour of catechin was investigated by cyclic voltammetry, revealing a well-defined anodic oxidation peak at +0.450 V, which was selected as the working potential for amperometry detection. The sensor exhibited a linear response towards catechin in the concentration range of 0-400 mg L-1 with excellent sensitivity. The limit of detection and limit of quantification were found to be 1.151 mg L-1 and 3.838 mg L-1, respectively. The analytical performance of the sensor was validated by comparison with high-performance liquid chromatography, showing strong agreement with a correlation coefficient of 0.994. Repeatability studies performed on 10 different tea samples demonstrated good precision, with coefficients of variation below 3%. The proposed micro-fabricated electrochemical sensor offers a simple, rapid and reliable alternative to conventional chromatographic methods for the determination of tea polyphenols and shows strong potential for on-site tea quality assessment and food analytical applications.
Polyphenols, screen-printed electrodes, cyclic voltammetry, amperometry
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